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KMID : 0350519920450010251
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Journal of Catholic Medical College
1992 Volume.45 No. 1 p.251 ~ p.261
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Morphometric Changes of Ileal Segment after Ileocystoplasty in Rats
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Abstract
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With the increasing use of bladder substtutes and augmentation cystoplsty with intestinal segments, it has become an interesting topic how the intestinal segments adapt to urinary millieu. Intestinal tract primarily has absorptive and secretory
functions. However, when it is grafted to urinary system, morphological and functional changes take place as time goes by. Although, the mucus secreted mostly by goblet cells in the transplanted intestinal mucosa elicts various problems in
patient.
This study was attempted to observe the objective and quantitative changes of the mucosal epithelium and the goblet cells of the grafted ileal segment in rats, which have undergone ileocystoplasty. The morphometric analysis was done using an
image
analyzer.
The animals. Weighing 280-330 g, were divided into 6 groups according to the post-operative days; control, in which no operation was done, and 1, 2, 4, 8 and 12 weeks groups after ileocystoplasty.
Distal ileum in the control and augmented bladers in the experimental groups were taken and prepared with hematoxylin-eosin(H & E) staining and periodic acid Shiff(PAS) reaction.
Histological changes of the ileocystoplasty specimens were observed by light microscopy. Changes in the grafted ileal segments, i.e., changes in areas of mucosal epithelium, number of goblet cells within 10* um* of mucosal epithelium, mean area
of
a
goblet cell, and ratio of areas of goblet cells and mucosal epithlium were evaluated bymorphometric analysis.
@ES The results were as follows:
@EN 1. Microscopically, transitional epithelium extended continuously over the anastomotic site towards the grafted portion, and muscular layer of the graft was showing marked hypertrophy. This finding began in the 4 weeks group.
2. In morphometric analysis of grafted ileal segments,
1) The area of mucosal epithelium was increased in the 1 week group(12.79*2.42*10* um*) compared with the control(10.64*1.17*10* um*)(P<0.05) . However,the differences between the control and other groups were statistically insignificant.
2) The number of goblet cells within 10* um* of mucosal epithelium in the 1 week group(1.45*0.16) was significantly increased compared with the control(1.10*0.31)(P<0.005). It was the highest in the 4 weeks group(2.17*0.33) and declined
thereafter.
3) The mean area of a goblet cell in each group was statistically insignificant compared with the cotrol.
4) The ratio of areas of goblet cells and mucosal epithelium in the 1 week group(24.33*3.67%) was signficantly increased compared with the control(17.22*3.33%)(P<0.005). It was the highest in the 4 weeks group(30.87*4.99%) and declined gradually
thereafter.
From the above results with morphometric analysis, we may conclude that mucus secretion peaks in the 4 weeks group after ileocystoplasty in rats and decreases thereafterl. It may be not due to the changes in the areas of goblet cells or mucosal
epithelium but due to the changes in number of the goblet cells.
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KEYWORD
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